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primescripttm double strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm double strand cdna synthesis kit
    Primescripttm Double Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+double+strand+cdna+synthesis+kit/PrimeScript+Double+Strand+cDNA+Synthesis+Kit/10__1007_slash_s10616___026___00970___4-87-66-72
    Average 96 stars, based on 1667 article reviews
    primescripttm double strand cdna synthesis kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: Diel rhythms shape viral community structure and activity across the host domains of life
    Article Snippet: This involved DNase treatment using recombinant deoxyribonuclease I (ribonuclease-free, 2270B, TaKaRa, Shiga, Japan) to remove residual DNA, followed by purification with the RNeasy MinElute Cleanup Kit (Qiagen, Maryland, USA). .. Finally, complementary DNA (cDNA) was synthesized from the purified RNA using the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa, 6111A). .. Metagenomic, viromic, and metatranscriptomic libraries were sequenced on the DNBSEQ-T5 platform (BGI-Shenzhen and BGI-Qingdao), generating 100/150 bp paired-end reads.

    Article Title: Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules
    Article Snippet: MRNA was extracted from total RNA for each group within 7 days using VAHTS ® mRNA Capture Beads (N401-02, Human, Vazyme). .. Double-stranded cDNA was synthesized from purified polyadenylated mRNA templates using the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa), following the manufacturer’s protocol. cDNA libraries for Illumina sequencing were prepared using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina ® (NR604-02, Vazyme). .. The library concentration was quantified using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA), and library fragment distribution was assessed with an Agilent 4200 TapeStation (Agilent Technologies, China).

    Article Title: Investigations on the effects of SH2B2 on Parkinson's disease based on its in vivo and in vitro neurotoxic model.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: MicroRNA-33a-5p alleviates cervical cancer by regulating PDE4B through FTO-mediated m6A demethylation
    Article Snippet: Background Cervical cancer (CVC), a common gynecological malignant tumor, ranks second among female malignant tumors worldwide.. However, the effects of miR-33a-5p, fat mass and obesity-associated protein (FTO), and phosphodiesterase 4B (PDE4B) on CVC are still unclear.. Therefore, this study aims to explore the mechanisms of miR-33a-5p, FTO, and PDE4B on CVC cells, and first verify their expression patterns in clinical CVC tissues.

    Purification:

    Article Title: Diel rhythms shape viral community structure and activity across the host domains of life
    Article Snippet: This involved DNase treatment using recombinant deoxyribonuclease I (ribonuclease-free, 2270B, TaKaRa, Shiga, Japan) to remove residual DNA, followed by purification with the RNeasy MinElute Cleanup Kit (Qiagen, Maryland, USA). .. Finally, complementary DNA (cDNA) was synthesized from the purified RNA using the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa, 6111A). .. Metagenomic, viromic, and metatranscriptomic libraries were sequenced on the DNBSEQ-T5 platform (BGI-Shenzhen and BGI-Qingdao), generating 100/150 bp paired-end reads.

    Article Title: Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules
    Article Snippet: MRNA was extracted from total RNA for each group within 7 days using VAHTS ® mRNA Capture Beads (N401-02, Human, Vazyme). .. Double-stranded cDNA was synthesized from purified polyadenylated mRNA templates using the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa), following the manufacturer’s protocol. cDNA libraries for Illumina sequencing were prepared using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina ® (NR604-02, Vazyme). .. The library concentration was quantified using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA), and library fragment distribution was assessed with an Agilent 4200 TapeStation (Agilent Technologies, China).

    Article Title: Investigations on the effects of SH2B2 on Parkinson's disease based on its in vivo and in vitro neurotoxic model.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: MicroRNA-33a-5p alleviates cervical cancer by regulating PDE4B through FTO-mediated m6A demethylation
    Article Snippet: Background Cervical cancer (CVC), a common gynecological malignant tumor, ranks second among female malignant tumors worldwide.. However, the effects of miR-33a-5p, fat mass and obesity-associated protein (FTO), and phosphodiesterase 4B (PDE4B) on CVC are still unclear.. Therefore, this study aims to explore the mechanisms of miR-33a-5p, FTO, and PDE4B on CVC cells, and first verify their expression patterns in clinical CVC tissues.

    cDNA Synthesis:

    Article Title: Diel rhythms shape viral community structure and activity across the host domains of life
    Article Snippet: This involved DNase treatment using recombinant deoxyribonuclease I (ribonuclease-free, 2270B, TaKaRa, Shiga, Japan) to remove residual DNA, followed by purification with the RNeasy MinElute Cleanup Kit (Qiagen, Maryland, USA). .. Finally, complementary DNA (cDNA) was synthesized from the purified RNA using the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa, 6111A). .. Metagenomic, viromic, and metatranscriptomic libraries were sequenced on the DNBSEQ-T5 platform (BGI-Shenzhen and BGI-Qingdao), generating 100/150 bp paired-end reads.

    Article Title: Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules
    Article Snippet: MRNA was extracted from total RNA for each group within 7 days using VAHTS ® mRNA Capture Beads (N401-02, Human, Vazyme). .. Double-stranded cDNA was synthesized from purified polyadenylated mRNA templates using the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa), following the manufacturer’s protocol. cDNA libraries for Illumina sequencing were prepared using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina ® (NR604-02, Vazyme). .. The library concentration was quantified using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA), and library fragment distribution was assessed with an Agilent 4200 TapeStation (Agilent Technologies, China).

    Article Title: Investigations on the effects of SH2B2 on Parkinson's disease based on its in vivo and in vitro neurotoxic model.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: The Molecular Landscape of CASTLE: A Rare Thymus-like Head and Neck Cancer
    Article Snippet: Subsequently, the NEBNext ® rRNA Depletion Kit v2 (New England Biolabs, Hitchin, UK) was used to selectively removed ribosomal RNAs (rRNAs), allowing us to focus on genic transcripts. .. The resulting RNA was reverse-transcribed into cDNA using the PrimeScriptTM Double Strand cDNA synthesis kit (Takara, Shiga, Japan). .. For sequencing library construction, the KAPA HyperPlus Kit (Roche) was used, followed by high-throughput sequencing on the NovaSeq Plus X PE150 platform (Illumina).

    Article Title: Insights into the biological features and improved diagnostics of adult acute myeloid leukemia via fusion genes identified through targeted next-generation sequencing
    Article Snippet: .. Briefly, first- and second-strand complementary DNA (cDNA) synthesis was conducted using the PrimeScriptTM Double Strand cDNA Synthesis Kit (Takara Bio Inc., Shiga, Japan). .. The double-stranded cDNA was then purified with Agencourt AMPure XP beads (Beckman Coulter, Inc., CA, USA), followed by end-repair, adenylation, and ligation using a universal barcode adapter, and subsequently amplified through seven cycles to generate mid-libraries.

    Article Title: Insights into the biological features and improved diagnostics of adult acute myeloid leukemia via fusion genes identified through targeted next-generation sequencing
    Article Snippet: .. Briefly, first- and second-strand complementary DNA (cDNA) synthesis was conducted using the PrimeScriptTM Double Strand cDNA Synthesis Kit (Takara Bio Inc., Shiga, Japan). .. The double-stranded cDNA was then purified with Agencourt AMPure XP beads (Beckman Coulter, Inc., CA, USA), followed by end-repair, adenylation, and ligation using a universal barcode adapter, and subsequently amplified through seven cycles to generate mid-libraries.

    Article Title: MicroRNA-33a-5p alleviates cervical cancer by regulating PDE4B through FTO-mediated m6A demethylation
    Article Snippet: Background Cervical cancer (CVC), a common gynecological malignant tumor, ranks second among female malignant tumors worldwide.. However, the effects of miR-33a-5p, fat mass and obesity-associated protein (FTO), and phosphodiesterase 4B (PDE4B) on CVC are still unclear.. Therefore, this study aims to explore the mechanisms of miR-33a-5p, FTO, and PDE4B on CVC cells, and first verify their expression patterns in clinical CVC tissues.

    Article Title: APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis.
    Article Snippet: .. RNA extraction was performed using RNAiso reagent (TaKaRa, Shiga, Japan), followed by reverse transcription with the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. ..

    RNA Sequencing:

    Article Title: Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules
    Article Snippet: MRNA was extracted from total RNA for each group within 7 days using VAHTS ® mRNA Capture Beads (N401-02, Human, Vazyme). .. Double-stranded cDNA was synthesized from purified polyadenylated mRNA templates using the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa), following the manufacturer’s protocol. cDNA libraries for Illumina sequencing were prepared using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina ® (NR604-02, Vazyme). .. The library concentration was quantified using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA), and library fragment distribution was assessed with an Agilent 4200 TapeStation (Agilent Technologies, China).

    RNA Extraction:

    Article Title: Investigations on the effects of SH2B2 on Parkinson's disease based on its in vivo and in vitro neurotoxic model.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: MicroRNA-33a-5p alleviates cervical cancer by regulating PDE4B through FTO-mediated m6A demethylation
    Article Snippet: Background Cervical cancer (CVC), a common gynecological malignant tumor, ranks second among female malignant tumors worldwide.. However, the effects of miR-33a-5p, fat mass and obesity-associated protein (FTO), and phosphodiesterase 4B (PDE4B) on CVC are still unclear.. Therefore, this study aims to explore the mechanisms of miR-33a-5p, FTO, and PDE4B on CVC cells, and first verify their expression patterns in clinical CVC tissues.

    Article Title: APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis.
    Article Snippet: .. RNA extraction was performed using RNAiso reagent (TaKaRa, Shiga, Japan), followed by reverse transcription with the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. ..

    Reverse Transcription:

    Article Title: The Molecular Landscape of CASTLE: A Rare Thymus-like Head and Neck Cancer
    Article Snippet: Subsequently, the NEBNext ® rRNA Depletion Kit v2 (New England Biolabs, Hitchin, UK) was used to selectively removed ribosomal RNAs (rRNAs), allowing us to focus on genic transcripts. .. The resulting RNA was reverse-transcribed into cDNA using the PrimeScriptTM Double Strand cDNA synthesis kit (Takara, Shiga, Japan). .. For sequencing library construction, the KAPA HyperPlus Kit (Roche) was used, followed by high-throughput sequencing on the NovaSeq Plus X PE150 platform (Illumina).

    Article Title: APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis.
    Article Snippet: .. RNA extraction was performed using RNAiso reagent (TaKaRa, Shiga, Japan), followed by reverse transcription with the PrimeScriptTM Double Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. ..

    Expressing:

    Article Title: MicroRNA-33a-5p alleviates cervical cancer by regulating PDE4B through FTO-mediated m6A demethylation
    Article Snippet: Background Cervical cancer (CVC), a common gynecological malignant tumor, ranks second among female malignant tumors worldwide.. However, the effects of miR-33a-5p, fat mass and obesity-associated protein (FTO), and phosphodiesterase 4B (PDE4B) on CVC are still unclear.. Therefore, this study aims to explore the mechanisms of miR-33a-5p, FTO, and PDE4B on CVC cells, and first verify their expression patterns in clinical CVC tissues.

    Quantitative RT-PCR:

    Article Title: MicroRNA-33a-5p alleviates cervical cancer by regulating PDE4B through FTO-mediated m6A demethylation
    Article Snippet: Background Cervical cancer (CVC), a common gynecological malignant tumor, ranks second among female malignant tumors worldwide.. However, the effects of miR-33a-5p, fat mass and obesity-associated protein (FTO), and phosphodiesterase 4B (PDE4B) on CVC are still unclear.. Therefore, this study aims to explore the mechanisms of miR-33a-5p, FTO, and PDE4B on CVC cells, and first verify their expression patterns in clinical CVC tissues.



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    Image Search Results


    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Journal: Frontiers in Neurology

    Article Title: Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms

    doi: 10.3389/fneur.2026.1793054

    Figure Lengend Snippet: Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Article Snippet: Total RNA was extracted from patients and their parents using the Qiagen RNA Preparation Kit, and complementary DNA (cDNA) was synthesized by reverse transcription using the PrimeScriptTM Strand cDNA Synthesis Kit/RT Master Mix (TAKARA).

    Techniques: Expressing, Variant Assay, Amplification